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	Provedor de dados Electron. J. Biotechnol. (1) Autor Li,Heng-xin (1) Wang,Dun (1) Yang,Ming-Ming (1) Zhang,Wei-Wei (1) Palavra-chave Bacillus subtilis (1) Biosynthesis (1) Integration (1) Pglv promoter (1) Pimelic acid (1) Single crossover event (1) Mais... Tipo do documento Journal article (1) Ano 2011 (1) País Chile (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 149 Primeira ... 1 2 3 4 5 6 7 8 ... Última Différenciation génétique de deux huîtres creuses Crassostrea gigas et Crassostrea angulata : apport des marqueurs microsatellites ArchiMer Huvet, Arnaud. Les définitions taxonomiques de Crassostrea angulata et Crassostrea gigas font l'objet de controverses. Les similitudes morphologiques et physiologiques ainsi que l'homogénéité des fréquences allozymiques entre populations des deux taxons orientent les auteurs vers un regroupement au sein d'une seule espèce. Récemment, une étude basée sur l'analyse par RFLP de l'ADN mitochondrial a révélé des différences permettant de distinguer C. angulata et C. gigas. La caractérisation par 4 marqueurs microsatellites de 12 populations des 2 taxons révèlent les points suivants : des déficits en hétérozygotes sont observés dans l'ensemble des populations, l'analyse de la variabilité inter-populations montre l'existence d'une structuration, l'analyse des distances... Tipo: Text Palavras-chave: Crassostrea gigas; Crassostrea angulata; Microsatellites; Différenciation. Ano: 1997 URL: http://archimer.ifremer.fr/doc/00033/14377/11667.pdf The use of microsatellite markers for parentage analysis in the Pacific cupped oyster, Crassostrea gigas (Thunberg) ArchiMer Boudry, Pierre; Collet, Bertrand; Kotoulas, Georgios; Magoulas, Antonio; Hervouet, Veronique; Bonhomme, Francois; Gerard, Andre. Highly polymorphic genetic markers can be useful tools for the analysis of parental contributions. The present paper reports 3 experiments using microsatellite markers to analyse parental contributions in controlled in vitro factorial crosses of the cupped oyster Crassostrea gigas. Parentage analysis was eased by the large polymorphism observed at the 3 loci studied. In a cross between 20 males and 20 females, a quarter of the progeny could be assigned to a given parent using a single microsatellite locus showing 28 alleles. In 3 crosses between 5 males and females, all the progeny could be assigned to a family by using 3 loci. In all cases, the combined genotyping at 2 loci allowed parentage to be detennined unambiguously. Despite the balanced gametic... Tipo: Text Palavras-chave: Crassostrea gigas; Microsatellites; Parental contributions; Crassostrea gigas; Microsatellites; Contributions parentales. Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/acte-2739.pdf Utilisation des marqueurs microsatellites pour l'étude des contributions parentales chez l'huître creuse Crassostrea gigas ArchiMer Boudry, Pierre; Collet, Bertrand; Kotoulas, G.; Hervouet, Veronique; Cornette, Florence; Bonhomme, Francois; Gerard, Andre. Numerous aquacultural species show very high fertilities. As a consequence, it is possible to produce, in hatcheries, a large amount of sibs from a small amount of parents. The sibs' genetic variability is, of course, directly linked to the number of parents involved, but it may be largely lowered by imbalanced parental contributions. Polymorphic genetic markers are very handy tools in the study of these phenomena in cultivated populations (Harris et al. 1991; Herbinger et al., 1996a; Garcia de Leon et al. 1998) or in natural populations (Ferguson et al, 1995; Kellog et al., 1995; Colbourne et al., 1996; Herbinger et al., 1996b). This document is a presentation of the results of a number of experiments, realised in the scope of the European program... Tipo: Text Palavras-chave: Microsatellites; Contributions parentales; Crassostrea gigas; Huîtres. Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/acte-3452.pdf Variable microsatellites in the Pacific Oyster Crassostrea gigas and other cupped oyster species ArchiMer Huvet, Arnaud; Boudry, Pierre; Ohresser, M; Delsert, Claude; Bonhomme, F. Source/description : Genomic DNA was extracted from a whole Pacific oyster (C. gigas) after grinding in liquid nitrogen. Purified DNA was then digested by a mix of 3 restriction enzymes (AluI, RsaI, HaeIII). DNA fragments ranging from 250 to 500 bp were size-selected by agarose gel electrophoresis and ligated into the dephosphorylated blunt-ended SmaI-linearised pBKS2 plasmid (Stratagene Cloning System, CA). White colonies (11,500) were screened by hybridisation with double-stranded DNA probes containing dinucleotide repeats. Poly(dAdC), poly(dAdG), poly(dAdT) (Pharmacia Biotech, Sweden) were mixed at equimolar ratio and radiolabelled by [-32P] alpha dATP and dCTP using a random priming labelling kit (Life Technologies, Germany). Filter... Tipo: Text Palavras-chave: Crassostrea gigas; Oyster; Microsatellites. Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-703.pdf Mitochondrial and nuclear DNA phylogeography of Crassostrea angulata, the Portuguese oyster endangered in Europe ArchiMer Huvet, Arnaud; Lapegue, Sylvie; Magoulas, A; Boudry, Pierre. The respective status of the Portuguese oyster, Crassostrea angulata, and the Pacific oyster, Crassostrea gigas, has long been a matter of controversy. Morphological and physiological similarities, homogeneity of allozyme allelic frequencies between populations of the two taxa and the demonstration of hybridization lead most authors to suggest that they should be regrouped within the same species. The risk of introgression and the present expansion of C. gigas aquaculture in Europe raises the question of the need for preservation of C. angulata in Europe, as only a few populations remain. We studied European and Asian populations of C. gigas and C. angulata using microsatellite and mitochondrial DNA markers to estimate their genetic diversity and... Tipo: Text Palavras-chave: Phylogeography; Population genetics; Microsatellites; Crassostrea gigas; Crassostrea angulata. Ano: 2000 URL: https://archimer.ifremer.fr/doc/2000/publication-707.pdf Microsatellite-associated heterosis in hatchery-propagated stocks of the shrimp Penaeus stylirostris ArchiMer Bierne, Nicolas; Bezuart, Ivan; Vonau, Vincent; Bonhomme, François; Bedier, Edouard. Correlation between DNA microsatellite heterozygosity and growth rate was investigated in two hatchery-propagated stocks of the shrimp Penaeus stylirostris, which had been genetically isolated from wild founders for 17 generations, and from each other for five more generations. presumed demographic history of these populations suggested that they were maintained at small effective population sizes. and this was confirmed by significant changes in allelic frequencies between the two stocks. Despite a small sample size (n = 60) and a small number of loci screened, a significant positive correlation was detected between microsatellite tri-locus heterozygosity and growth rate in one of the two stocks (r = 0.3, P = 0.02). In the other stock (n = 48), the... Tipo: Text Palavras-chave: Penaeus stylirostris; Heterozygosity; Marker associated heterosis; Inbreeding depression; Microsatellites. Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-753.pdf Microsatellite analysis of 6-hour-old embryos reveals no preferential intraspecific fertilization between cupped oysters Crassostrea gigas and Crassostrea angulata ArchiMer Huvet, Arnaud; Balabaud, K; Bierne, Nicolas; Boudry, Pierre. Experimental examination of reproductive isolation is the first step in understanding li,hybridization processes. Here, we studied preferential fertilization between 2 cupped oyster taxa, Crassostrea angulata and Crassostrea gigas, as a potential prezygotic reproductive isolation. Early examination of sperm competition is now possible by molecular analysis of oyster embryos. This avoids the confounding effect of differential mortality during the larval stage. Six hundred embryos were sampled from 2 crosses. Three microsatellite loci were enough to determine without ambiguity the taxa of contributing sires of embryos. No evidence of preferential fertilization between gametes from the same taxa was shown. A significantly higher contribution of the C. gigas... Tipo: Text Palavras-chave: Paternal contribution; Microsatellites; In vitro crossings; Hybridization; Crassostrea angulata; Crassostrea gigas. Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-436.pdf Characterization of Brazilian soybean cultivars using microsatellite markers Genet. Mol. Biol. Priolli,Regina Helena Geribello; Mendes-Junior,Celso Teixeira; Arantes,Neylson Eustáquio; Contel,Eucleia Primo Betioli. Microsatellite markers or SSR (Simple Sequence Repeats) have proved to be an excellent tool for cultivar identification, pedigree analysis and the evaluation of genetic distance among organisms. Soybean cultivars have been characterized mainly by morphological and biochemical traits. However, these traits have not been sufficient to characterize the large number of cultivars eligible to receive protection under the Brazilian Cultivar Protection Act. In order to define new soybean cultivar markers, the alleles of twelve SSR loci of 186 Brazilian soybean cultivars were studied by estimating the variation in their size range and their respective frequencies. On average, 5.3 alleles per locus were detected, with a mean genetic diversity of 0.64 ± 0.12. These... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max (L.) Merrill; Simple sequence repeat; Microsatellites; Molecular markers; Soybean elite cultivars. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200012 Evaluation of polymorphism in ten microsatellite loci in Uruguayan sheep flocks Genet. Mol. Biol. Tomasco,Ivanna; Wlasiuk,G.; Lessa,E.P.. The allele frequencies of 10 microsatellite loci previously described for sheep as BM1314, BM6526, OarFCB128, OarHH64, OarCP20, OarHH47, OarFCB48, OarHH35, OarHH72 and BM2508 were estimated for the Uruguayan flocks. A representative sample of 101 individuals composed by the two predominant breeds (76% Corriedale and 24% Australian Merino) was used. The sample did not show a significant tendency towards substructuring, in spite of presenting some significantly different allele frequencies between races. The Corriedale sample presents three loci in which the presence of null alleles is possible. The markers were highly variable, showing between 7 and 15 alleles each. The Polymorphism Information Content Index ranged from 0.63 to 0.87 and the Exclusion... Tipo: Info:eu-repo/semantics/article Palavras-chave: Sheep; Polymorphism; Microsatellites. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100008 Microsatellites in Portuguese autochthonous horse breeds: usefulness for parentage testing Genet. Mol. Biol. Luís,C.; Cothran,E.Gus; Oom,M.M.. The use of DNA technology for parentage testing is increasing every day. Most laboratories have improved their exclusion probabilities (PE) by the addition of DNA microsatellite loci to standard blood-typing results. The efficacy of each locus depends on the number of alleles detected and their frequencies in the breeds tested. Here we analyzed the usefulness of six microsatellites for routine parentage testing in three Portuguese autochthonous horse breeds: Lusitano, Sorraia and Garrano. The DNA loci analyzed - ASB2, HMS3, HMS7, HTG4, HTG10, and VHL20 - were chosen based upon the polymorphism detected in other breeds with Iberian horse influence. The estimated probability of exclusion of wrongly named parents (PE) was high, with values ranging from 88.5%... Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; DNA; Portuguese horse breeds; Parentage testing. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200003 Diversité génétique et dynamique du recrutement chez l'huître plate Ostrea edulis L. 2002 ArchiMer Taris, Nicolas. Cette étude aborde la diversité génétique d'une espèce de bivalve marin, Ostrea edulis et plus particulièrement les interactions existant entre génétique (flux de gènes) et démographie (dynamique de reproduction) à l'échelle de la population. Elle repose sur l'utilisation de marqueurs microsatellites pour tenter de comprendre les facteurs participant à la mise ne place d'une structuration génétique au sein de laquelle peuvent être observés des phénomènes de déficit en hétérozygotes et de ration positive entre hétérozygotie et valeur sélective. L'un des objectifs est en particulier de tester l'hypothèse de consanguinité à l'échelle locale. Pour cela, nous avons étudier la structure génétique de cohortes d'huîtres plates au cours d'une saison de reproduction... Tipo: Text Palavras-chave: Ostrea edulis; Génétique des Populations; Microsatellites; Diversité génétique; Dynamique de recrutement. Ano: 2002 URL: http://archimer.ifremer.fr/doc/00033/14419/11714.pdf Comparative genetic structure in pines: evolutionary and conservation consequences RChHN DELGADO,PATRICIA; CUENCA,ARGELIA; ESCALANTE,ANA E.; MOLINA-FREANER,FRANCISCO; PIÑERO,DANIEL. Pines have been the focus of several studies that estimate population genetic parameters using both allozymes and chloroplast single sequence repeats (SSRs). Also, the genus has also been recently studied using molecular systematics so that we now have a more clear understanding of their evolutionary history. With this background we studied comparatively the genetic structure in pines. Expected heterozygosity is particularly constant with a 99 % confidence interval between 0.19 and 0.23 in species that have been studied until now using allozymes. There is a significant proportion of species (9/41) that show high population differentiation estimates (Fst = or larger than 0.15) and five of these have large and wingless seeds probably associated with low... Tipo: Journal article Palavras-chave: Pinus; Genetic structure; Outcrossing rates; Microsatellites; Allozymes. Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2002000100004 Evaluation of nine microsatellite loci and misidentification paternity frequency in a population of Gyr breed bovines Braz. J. Vet. Res. Anim. Sci. Curi,Rogério Abdallah; Lopes,Catalina Romero. Paternity misidentification is harmful due to the reduction in annual genetic earnings of the population and because it endangers an efficient genetic improvement program. The objectives of the present study was to evaluate nine microsatellites in Paternity Testing and to investigate misidentification paternity frequency in families of Gyr breed bovines population. In the present experiment blood samples from forty Gir breed families ( bull / cow / calf ), registered pure breed in the Zebu Breeders Brazilian Association (ABCZ) were used. The most part of the microsatellites used in this work were recommended by the International Society of Animal Genetics (ISAG). The genomic DNA extraction was performed from whole blood samples. The microsatellites... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA; Microsatellites; Paternity; Bovine; Gyr. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000300004 Genetic differentiation in geographically close populations of the water rat Nectomys squamipes (Rodentia, Sigmodontinae) from the Brazilian Atlantic Forest Genet. Mol. Biol. Maroja,L.S.; Almeida,F.C.; Seuánez,H.N.. We examined the genetic structure and the effects of a bottleneck in populations of the water rat Nectomys squamipes, a primary host of Schistosoma mansoni. Eight microsatellite loci were studied in 7 populations from the Sumidouro region of the Brazilian state of Rio de Janeiro. Our data, covering a four-year period during which a bottleneck occurred, revealed substantial variation (6-31 alleles per locus) and high levels of both observed (0.718-0.789) and expected (0.748-0.832) heterozygosity. Most populations were in Hardy-Weinberg equilibrium without linkage disequilibrium between loci. Overall average genetic differentiation between populations (estimated with the F ST (q) and R ST (r) analogues was 0.037 for q and 0.060 for r. There was significant... Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; Migration; Population genetics; Schistosoma mansoni host; Sigmodontinae. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400002 Isolation of (GA)n microsatellite sequences and description of a predicted MADS-box sequence isolated from common bean (Phaseolus vulgaris L.) Genet. Mol. Biol. Yaish,M.W.F.; Pérez de la Vega,M.. The isolation of (GA)n microsatellites using a highly microsatellite-enriched library is described for the first time in common bean (Phaseolus vulgaris L.). A relatively simple and effective method to isolate DNA repeats from microsatellite-enriched libraries based on hybridization-capture of repeat regions using biotin-conjugated oligonucleotids and non-radioactive colony hybridization was carried out. PCR products from 200 to 800 bp were obtained and cloned. Of the 60 clones sequenced, 21 yielded (GA)n microsatellites with n values equal or higher than six. These (GA)n microsatellite-containing loci could be useful for further genetic mapping studies. A (GA)n microsatellite linked to a putative MADS-box gene was identified. This sequence, which... Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris L.; Genetic markers; Microsatellites; MADS-box; Transcriptional factor. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300019 Analyse du succès reproducteurs chez des populations naturelles et d'écloserie d'huître plate Ostrea edulis L. ArchiMer Lallias, Delphine. Cette étude vise à approfondir la connaissance de la biologie de la reproduction de l'huître plate Ostrea edulis afin de mieux comprendre comment les caractéristiques de la biologie de reproduction de l'espèce peuvent rendre compte des phénomènes de déficits en hétérozygote et de relation positive entre hétérozygotie et croissance. En particulier, elle vise à analyser le succès reproducteur en milieu naturel et dans les populations maintenues en écloserie. Cette étude repose sur l'utilisation de marqueurs microsatellites qui, du fait de leur fort polymorphisme, représentent des outils puissants d'assignation de parenté. Une première partie de l'étude a consisté à analyser les pontes issues de femelles incubantes prélevées dans le milieu naturel. Le nombre... Tipo: Text Palavras-chave: Ostrea edulis; Microsatellites; Biologie de la reproduction; Variance du succès reproducteur. Ano: 2003 URL: http://archimer.ifremer.fr/doc/00370/48167/48281.pdf Diversité et différentiation génétique chez l'huître plate européenne : de la population à l'individu (Actes) ArchiMer Boudry, Pierre; Launey, Sophie; Diaz-almela, A.; Naciri-graven, Yamama; Ledu, Christophe; Mira, S.; Taris, Nicolas; Bonhomme, Francois; Lapegue, Sylvie. PCR-based DNA markers enable the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European fiat oyster (Ostrea edulis) populations aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species. An Atlantic/Mediterranean differentiation pattern was observed. Similar patterns of variation were found using allozymes, microsatellites and mitochondrial DNA, aIl which support an isolation by distance model. High variability was observed in the range of diversity between populations using a mitochondrial marker, a reflection of small effective population sizes in sorne locations. A 10fold quantitative difference was observed in Fst,... Tipo: Text Palavras-chave: Recruitment; Diversity; Molecular markers; Population genetics; Ostrea edulis; Flat oyster; Dynamique de recrutement; Diversité génétique; Microsatellites; Marqueurs moléculaires; Génétique des populations; Ostrea edulis; Huître plate. Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-2851.pdf Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses PAB Koehler-Santos,Patrícia; Dornelles,Ana Lúcia Cunha; Freitas,Loreta Brandão de. The objective of this work was to characterize mandarin (Citrus spp.) germplasm from Southern Brazil by morphological and molecular analyses. Thirty seven cultivars from 34 distinct mandarin varieties were evaluated by morphological and agronomic traits of leaves, flowers and fruits, and by microsatellite markers. The morphological and agronomic characteristics suggested that almost all varieties can be produced for commercial use, and some, as the Satsuma variety, are recommended for breeding programs. Pooled DNA samples from 1-5 plants belonging to each cultivar were tested. Eight of the nine primers detected polymorphisms. Specific markers were found for some accessions. The dendrogram constructed with the morphological results divided the 37 cultivars... Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus; Genetic variation; Microsatellites; Breeding methods. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000700003 Genetic variability in Amerindian populations of Northern Argentina Genet. Mol. Biol. Dejean,Cristina B.; Crouau-Roy,Brigitte; Goicoechea,Alicia S.; Avena,Sergio A.; Carnese,Francisco R.. The allelic variability of four dinucleotide microsatellites located in the HLA region (MOGc, D6S265, MIB, and TNFa) was analyzed in 67 individuals representing three Amerindian populations of the Argentine Gran Chaco: Toba, Wichi and Chorote. Genomic DNA was prepared from peripheral blood and DNA was extracted using the standard phenol-chloroform procedure. Alleles were identified by PCR, using an end-labelled reverse oligonucleotide primer (fluorescent 6 - Fam labeling). Despite the low number of samples studied, a high level of gene diversity was observed in each population and for each locus. Moreover, the mean number of alleles was 7.7, 5.3, 10.0, and 7.0 at loci MOGc, D6S265, MIB and TNFa, respectively. Differentiation tests between pairs of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aboriginal Argentinians; Microsatellites; Intra- and inter-populational variation. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400004 Domestication en phase larvaire chez l'huître creuse Crassostrea gigas. Effets sélectifs des conditions environnementales appliquées en écloseries sur la diversité génétique ArchiMer Sauvage, Christopher. L'huître creuse Crassostrea gigas est, aujourd'hui, la première espèce ostréicole française avec une production de 140000 tonnes par an. Parallèlement au recrutement naturel, les écloseries produisent aujourd'hui 20% du naissain mis en élevage. Cette production est réalisée dans des conditions assurant une productivité maximale (température supérieure à 25°C, ration alimentaire optimale et tamisage sélectif). Peu de travaux se sont intéressés à l'impact génétique de ces pratiques d'élevage intensif au stade larvaire. Notre étude porte sur l'effet de la température appliquée lors de l'élevage larvaire sur la croissance et la survie et leurs composantes génétiques. Un croisement factoriel de 4 femelles et 12 mâles a été réalisé, en choisissant les géniteurs... Tipo: Text Palavras-chave: Crassostrea gigas; Ecloseries; Croissance; Survie; Température; Microsatellites; Larves; Sélection. Ano: 2004 URL: http://archimer.ifremer.fr/doc/00033/14418/11713.pdf Registros recuperados: 149 Primeira ... 1 2 3 4 5 6 7 8 ... Última

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